Given below are two statements :Statement I: The DNA fragments extracted fromgel electrophoresis can be used in construction ofrecombinant DNA.Statement II: Smaller size DNA fragments areobserved near anode while larger fragments arefound near the wells in an agarose gel.In the light of the above statements, choose the mostappropriate answer from the options given below:
Answer
Detailed Explanation: Gel Electrophoresis Statements
Statement I:
“The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.”
Correct. DNA fragments separated by gel electrophoresis can be visualized under UV light after staining (e.g., with ethidium bromide). The desired DNA band is cut from the gel, purified, and used to construct recombinant DNA by ligation into vectors.
Statement II:
“Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.”
Correct. During agarose gel electrophoresis, DNA fragments migrate based on size. Smaller DNA fragments move faster and travel towards the anode (positive electrode), while larger fragments move slower and remain near the wells (close to the cathode).
Conclusion:
Both Statement I and Statement II are correct.
Final Answer:
(1) Both statement I and statement II are correct
Summary Table
| Statement | Description | Correct? |
|---|---|---|
| I | DNA fragments from gel electrophoresis used in recombinant DNA construction | ✔ Yes |
| II | Smaller DNA fragments move near anode; larger fragments near wells in agarose gel | ✔ Yes |
| Final Answer | (1) Both I and II are correct | ✔ Correct |
This explanation clarifies the behavior of DNA fragments during gel electrophoresis and identifies the correct statements.