
Answer
DNA Amplification in Polymerase Chain Reaction (PCR)
In a Polymerase Chain Reaction (PCR), each cycle of amplification doubles the amount of DNA. This doubling leads to an exponential increase in DNA molecules.
Starting with 1 molecule of DNA:
- After 1 cycle → 2 molecules
- After 2 cycles → 4 molecules
- After 3 cycles → 8 molecules
- After n cycles →
2nmolecules
This pattern follows the exponential formula:
Amount of DNA after n cycles = 2n
Where:
n = number of amplification cycles
2n = fold increase in DNA quantity
